@article{83956,
  author = {V{\'\i}tor Trovisco and Katsiaryna Belaya and Dmitry Nashchekin and Uwe Irion and George Sirinakis and Richard Butler and Jack Lee and Elizabeth Gavis and Daniel St Johnston},
  title = {bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring.},
  abstract = {<p>bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot(2A2), which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110-120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.</p>
},
  year = {2016},
  journal = {Elife},
  volume = {5},
  month = {10/2016},
  issn = {2050-084X},
  doi = {10.7554/eLife.17537},
  language = {eng},
}