@article{83941,
  author = {Joel Tamayo and Takamasa Teramoto and Seema Chatterjee and Traci Tanaka Hall and Elizabeth Gavis},
  title = {The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition.},
  abstract = {<p>The Drosophila hnRNP F/H homolog, Glorund (Glo), regulates nanos mRNA translation by interacting with a structured UA-rich motif in the nanos 3{\textquoteright} untranslated region. Glo regulates additional RNAs, however, and mammalian homologs bind G-tract sequences to regulate alternative splicing, suggesting that Glo also recognizes G-tract RNA. To gain insight into how Glo recognizes both structured UA-rich and G-tract RNAs, we used mutational analysis guided by crystal structures of Glo{\textquoteright}s RNA-binding domains and identified two discrete RNA-binding surfaces that allow Glo to recognize both RNA motifs. By engineering Glo variants that favor a single RNA-binding mode, we show that a subset of Glo{\textquoteright}s functions in\&nbsp;vivo is mediated solely by the G-tract binding mode, whereas regulation of nanos requires both recognition modes. Our findings suggest a molecular mechanism for the evolution of dual RNA motif recognition in Glo that may be applied to understanding the functional diversity of other RNA-binding proteins.</p>
},
  year = {2017},
  journal = {Cell Rep},
  volume = {19},
  pages = {150-161},
  month = {04/2017},
  issn = {2211-1247},
  doi = {10.1016/j.celrep.2017.03.022},
  language = {eng},
}