@article{203391,
  author = {Shane Breznak and Yingshi Peng and Limin Deng and Noor Kotb and Zachary Flamholz and Ian Rapisarda and Elliot Martin and Kara LaBarge and Dan Fabris and Elizabeth Gavis and Prashanth Rangan},
  title = {H/ACA snRNP-dependent ribosome biogenesis regulates translation of polyglutamine proteins.},
  abstract = {<p>Stem cells in many systems, including germline stem cells (GSCs), increase ribosome biogenesis and translation during terminal differentiation. Here, we show that the H/ACA small nuclear ribonucleoprotein (snRNP) complex that promotes pseudouridylation of ribosomal RNA (rRNA) and ribosome biogenesis is required for oocyte specification. Reducing ribosome levels during differentiation decreased the translation of a subset of messenger RNAs that are enriched for CAG trinucleotide repeats and encode polyglutamine-containing proteins, including differentiation factors such as RNA-binding Fox protein 1. Moreover, ribosomes were enriched at CAG repeats within transcripts during oogenesis. Increasing target of rapamycin (TOR) activity to elevate ribosome levels in H/ACA snRNP complex-depleted germlines suppressed the GSC differentiation defects, whereas germlines treated with the TOR inhibitor rapamycin had reduced levels of polyglutamine-containing proteins. Thus, ribosome biogenesis and ribosome levels can control stem cell differentiation via selective translation of CAG repeat-containing transcripts.</p>
},
  year = {2023},
  journal = {Science advances},
  volume = {9},
  pages = {eade5492},
  month = {06/2023},
  issn = {2375-2548},
  doi = {10.1126/sciadv.ade5492},
  language = {eng},
}